ABSTRACT
BACKGROUND/OBJECTIVES: Vascular dementia (VaD) caused by reduced blood supply to the brain manifests as white matter lesions accompanying demyelination and glial activation. We previously showed that arabinoxylan consisting of arabinose and xylose, and arabinose itself attenuated white matter injury in a rat model of VaD. Here, we investigated whether larch arabinogalactan (LAG) consisting of arabinose and galactose could also reduce white matter injury. MATERIALS/METHODS: We used a rat model of bilateral common carotid artery occlusion (BCCAO), in which the bilateral common carotid arteries were exposed and ligated permanently with silk sutures. The rats were fed a modified AIN-93G diet supplemented with LAG (100 mg/kg/day) for 5 days before and 4 weeks after being subjected to BCCAO. Four weeks after BCCAO, the pupillary light reflex (PLR) was measured to assess functional consequences of injury in the corpus callosum (cc). Additionally, Luxol fast blue staining and immunohistochemical staining were conducted to assess white matter injury, and astrocytic and microglial activation, respectively. RESULTS: We showed that white matter injury in the the cc and optic tract (opt) was attenuated in rats fed diet supplemented with LAG. Functional consequences of injury reduction in the opt manifested as improved PLR. Overall, these findings indicate that LAG intake protects against white matter injury through inhibition of glial activation. CONCLUSIONS: The results of this study support our hypothesis that cell wall polysaccharides consisting of arabinose are effective at protecting white matter injury, regardless of their origin. Moreover, LAG has the potential for development as a functional food to prevent vascular dementia.
Subject(s)
Animals , Rats , Hypoxia , Arabinose , Brain , Carotid Arteries , Carotid Artery, Common , Cell Wall , Corpus Callosum , Dementia, Vascular , Demyelinating Diseases , Diet , Functional Food , Galactose , Larix , Models, Animal , Optic Tract , Polysaccharides , Reflex , Silk , Sutures , White Matter , XyloseABSTRACT
The needled leaves of three conifer species were collected in Mt. Taehwa during different seasons of the year. Total 59 isolates and 19 species of endophytic fungi were isolated from the leaves and identified using morphological and molecular characteristics. As a result, Shannon index was different in its host plant; Larix kaempferi had a highest value of species diversity. According to the sampling season, 9 species of 19 species were isolated during fall season. The results suggest that the existing of host plant and sampling season are major factors of distribution of endophytic fungi.
Subject(s)
Tracheophyta , Endophytes , Fungi , Korea , Larix , Plants , SeasonsABSTRACT
The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the most extensively used methods in molecular cytogenetics producing chromosome landmarks, fluorescence in situ hybridization (FISH) has significantly facilitated karyotype construction, especially in species with morphologically similar chromosomes. This study developed a simple but effective use of combinatorial labeling probes to distinguish chromosomes of Larix principis-rupprechtii by multicolor FISH. Three highly repetitive sequences in Larix were selected: 25S rDNA hybridized at all of the secondary constrictions of two pairs of metacentrics and the largest pair of submetacentrics; 5S rDNA hybridized at subtelomeric sites of one pair of metacentrics that also harboured 25S rDNA on different arms; LPD family sequences are tandem repeats hybridized at proximal regions of 22 chromosomes. The three different probes were labeled with only two different labels, hybridized to metaphase chromosomes of Larix principis-rupprechtii, simultaneously visualized, and unequivocally distinguished in a single FISH experiment. These multicolor FISH marks largely improved the karyotype analysis of Larix principis-rupprechtii.
Subject(s)
Chromosome Mapping , In Situ Hybridization, Fluorescence , Larix/genetics , Chromosomes, Plant/genetics , DNA, Ribosomal/genetics , Karyotyping , Larix/physiology , RNA, Plant/genetics , /geneticsABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents in bark of Larix olgensis var. koreana.</p><p><b>METHOD</b>The compounds were isolated with silica gel column chromatography and their structures were elucidated on the basis of spectral analysis (IR, EI-MS, 1H-NMR, 13C-NMR).</p><p><b>RESULT</b>Eight compounds were isolated and identified as isopimaric acid (I), beta-sitosterol (II), 24R,5alpha-stigmast-3,6-dione (III), larixol (IV), ferulic acid (V), lariciresinol (VI), secroisolariciresinol (VII) and isolariciresinol (VIII).</p><p><b>CONCLUSION</b>All the compounds were isolated from this plant for the first time.</p>
Subject(s)
Carboxylic Acids , Chemistry , Furans , Chemistry , Larix , Chemistry , Lignans , Chemistry , Phenanthrenes , Chemistry , Plant Bark , Chemistry , Plants, Medicinal , Chemistry , Sitosterols , ChemistryABSTRACT
<p><b>OBJECTIVE</b>The study is the research on the preparation of arabinogalactan (AG) dropping pills and the releasing mechanism.</p><p><b>METHOD</b>Use the orthogonal test to find out the best way to produce and advance the preparation of AG dropping pills, analysis according to the chart and DSC to find the releasing mechanism.</p><p><b>RESULT</b>The best preparation conditions are: the liquid of AG is at 75 degrees C, the temperature above the polydimethls iloxane is 30 degrees C, the distance to the frizzed liquid is 6 cm, the speed of the liquid is 30 drop x min(-1). The chart and DSC suggest: The solid disoperation of AG-PREG 4000 the complex is in a certain form which made the melting point decreased obviously, so as to increase the solution of the medicine in carrier to increase the releasing speed.</p><p><b>CONCLUSION</b>The best preparation is reasonable, AG and carrier become a form, the melting point is low, it can release fast.</p>
Subject(s)
Dimethylpolysiloxanes , Drug Carriers , Drugs, Chinese Herbal , Galactans , Larix , Chemistry , Plants, Medicinal , Chemistry , Polyethylene Glycols , Solubility , Technology, Pharmaceutical , Methods , TemperatureABSTRACT
Six strains of Sparassis crispa such as S. crispa DUM-01, DUM-02, DUM-03, DUM-04, DUM-05, and DUM-06 were tested for their mycelial growth on 6 different kinds of sawdust media and primordial formation on 10 different compositions of larch sawdust media. The highest mycelial growth was recorded on the larch sawdust. Of the 6 strains of S. crispa, S. crispa DUM-04 recorded the favorable formation of primordia. The primordial formation of S. crispa DUM-04 was more favorable on L-3 medium than 9 kinds of larch sawdust media. When S. crispa DUM-04 was cultured on the media of larch sawdust+pine sawdust, the formation of its fruiting bodies was more outstanding on the media of larch sawdust+pine sawdust than those of larch sawdust.